Effects of Qingshen Granules on the Oxidative Stress-NF/kB Signal Pathway in Unilateral Ureteral Obstruction Rats.

Hua Jin,Lei Zhang,Yiping Wang,Dong Wang

doi:10.1155/2018/4761925

Abstract

Background. The activation of NF-kappa B (NF/kB) signaling pathway plays an important role in the process of epithelial-mesenchymal transition (EMT) and renal interstitial fibrosis (RIF) in renal tubules. The process of oxidative stress reaction in kidney is via excessive reactive oxygen species (ROS) production to activate NF/kB signaling pathway. Qingshen Granule (QSG) is an effective Chinese formula utilized to treat chronic renal failure. Previous studies confirmed that QSG could inhibit RIF in unilateral ureteral obstruction (UUO) rats. In this study, we used UUO rats to investigate the effects of QSG on oxidative stress and the activation of NF/kB signaling. Seventy male Sprague-Dawley (SD) rats were randomly divided into a sham group, UUO model group, Qingshen Granules (QSG) high-dose, medium-dose, and low-dose groups, PDTC group, and candesartan group (10 rats in each group). Our study demonstrated that oxidative stress-NF/kB signal pathway contributed to the formation of UUO renal interstitial fibrosis. QSG may protect against RIF by inhibiting the oxidative stress-NF/kB signal pathway, reducing inflammation, and improving renal tubular EMT.

Highlights

Chronic kidney disease (CKD) often progresses slowly which leads to an extensive renal interstitial fibrosis (RIF) in renal tissue and into end-stage renal disease (ESRD) [1,2,3]
Previous studies have shown that the activation of the NFkappa B (NF/kB) signaling pathway plays an important role in the process of epithelialmesenchymal transition (EMT) and RIF in renal tubules
In the Qingshen Granules (QSG), Pyrrolidine dithiocarbamate (PDTC), and candesartan groups, the levels of MDA and reactive oxygen species (ROS) were lower than in the model group (P < 0.01); the lowest levels were found in the QSG medium-dose group (P < 0.05)

Summary

Introduction

Chronic kidney disease (CKD) often progresses slowly which leads to an extensive RIF in renal tissue and into end-stage renal disease (ESRD) [1,2,3]. Previous studies have shown that the activation of the NFkappa B (NF/kB) signaling pathway plays an important role in the process of EMT and RIF in renal tubules. The activation of the NF/kB signaling pathway by an oxidative stress reaction in the kidney via excessive ROS production leads to renal inflammatory immune response, tubular EMT, extracellular matrix (ECM) accumulation, and, RIF. The activation of NF-kappa B (NF/kB) signaling pathway plays an important role in the process of epithelialmesenchymal transition (EMT) and renal interstitial fibrosis (RIF) in renal tubules. We used UUO rats to investigate the effects of QSG on oxidative stress and the activation of NF/kB signaling. Our study demonstrated that oxidative stress-NF/kB signal pathway contributed to the formation of UUO renal interstitial fibrosis. QSG may protect against RIF by inhibiting the oxidative stress-NF/kB signal pathway, reducing inflammation, and improving renal tubular EMT

Methods

Results

Discussion

Conclusion