Abstract
An experiment was conducted to investigate the effect of dietary pyridoxine on the gene expression of appetite-regulating peptides in the hypothalamus and gastrointestinal tract of rabbits. Thirty-two rabbits were randomly divided into 2 treatments for 8 weeks (16 replicates/group and 1 rabbit/replicate). The treatments were fed a basal diet (control, measured pyridoxine content is 4.51 mg/kg) and the basal diet with a pyridoxine supplementation at 10 mg/kg (pyridoxine, measured pyridoxine content is 14.64 mg/kg). The results showed that dietary pyridoxine did not significantly alter the mRNA levels of neuropeptide Y, agouti related peptide, pro-opiomelanocortin and cocaine, amphetamine regulated transcript, peptide YY and cholecystokinin in arcuate nucleus, peptide YY in jejunum and ileum, and cholecystokinin in duodenum, jejunum and ileum (P > 0.05). Compared with the control, the mRNA levels of corticotropin-releasing hormone and melanocortin 4 receptor in paraventricular nuclei and peptide YY in duodenum were significantly decreased after pyridoxine treatment (P 0.05). In conclusion, the appetite genes of melanocortin 4 receptor and corticotropin-releasing hormone in paraventricular nuclei and peptide YY in duodenum are involved in the pyridoxine-caused hyperphagia.
Highlights
The hypothalamus is a crucial region for integrating signal from central and peripheral pathways and plays a major role in appetite regulation
The results showed that dietary pyridoxine did not significantly alter the mRNA levels of neuropeptide Y, agouti related peptide, pro-opiomelanocortin and cocaine, amphetamine regulated transcript, peptide YY and cholecystokinin in arcuate nucleus, peptide YY in jejunum and ileum, and cholecystokinin in duodenum, jejunum and ileum (P > 0.05)
No significant difference was observed in agouti related peptide (AgRP), neuropeptide Y (NPY), POMC and cocaine and amphetamine regulated transcript (CART) mRNA levels (Figure 1(a)) in arcuate nucleus (ARC) between 2 groups (P > 0.05), corticotropin-releasing hormone (CRH) and melanocortin 4 receptor (MC4R) mRNA levels in paraventricular nuclei (PVN) were significantly decreased after pyridoxine
Summary
The hypothalamus is a crucial region for integrating signal from central and peripheral pathways and plays a major role in appetite regulation. The NPY/AgRP and POMC/CART neurones perform a primary sensory function in the neural regulation of appetite. These “first order” neurones relay metabolic information to other “second order” neuronal populations located elsewhere in the hypothalamus such as the corticotropin-releasing hormone (CRH) and melanocortin 4 receptor (MC4R) in the paraventricular nuclei (PVN) [4]. Gut hormones act to modulate digestion and absorption of nutrients They act as neurotransmitters within the central nervous system to control food intake. We investigated the effect of pyridoxine on the gene expression of food intake regulatory peptides in the hypothalamus and gastrointestinal tract of Rex rabbits. The results will be useful to understand the progress of pyridoxine regulating energy homeostasis
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