Abstract
The principle products of arachidonic acid (AA) in platelets are hydroxylated fatty acids and thromboxane B2(TXB2). Prostaglandin D2(PGD2) has been considered to be a nonenzymatic degradation product of prostaglandin H2 formed in the presence of plasma albumin. Using 14C AA as substrate and thin layer and silicic acid chromatography, we have demonstrated PGD2 synthesis by washed (albumin-free) human platelets. The identity of PGD2 was confirmed by gas chromatography-mass spectrometry. In platelets lysed by freezing and thawing synthesis of TXB2 and PGD2 was approximately equal and equally inhibited by pyrazolones.Synthesis of PGD2 by platelets is enzymatic and may contribute to bronchoconstrictor, vasomotor, and inflammatory effects induced by platelet aggregation. Pyrazolones appear to inhibit cyclooxygenase activity rather than the breakdown of cyclic endoperoxides as previously postulated.
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