Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts

Jian Chen,Jianquan He,Hui Huang,Zhang Xin

doi:10.4236/jbise.2017.101003

Abstract

The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E2) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E2, PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.

Highlights

Bone through this continuous dynamic remodeling provides structural integrity, skeletal strength, and a reservoir for hematopoiesis
The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model
We investigated the effect of pulsed electromagnetic fields of different frequencies on the gene expression of RANK and NFATc1 in rat osteoclasts co-cultured with osteoblasts and researched the molecular mechanisms underlying the effects of PEMFs on osteoclasts co-cultured with osteoblasts in vitro

Summary

Introduction

Bone through this continuous dynamic remodeling provides structural integrity, skeletal strength, and a reservoir for hematopoiesis. Osteoblasts and osteoclasts, maintain bone homeostasis by balancing each other’s function [1] [2]. Increased osteoclast numbers and activity cause osteoporosis and periodontal disease. This bone remodeling process is regulated by numerous lo-. J. Chen et al 22 cal or systemic factors, which are secreted by either osteoblast-lineage cells and influence differentiation and activity of both osteoblasts and osteoclasts or are released by osteoclasts to regulate osteoblast activity [3]. Direct contacts between osteoclasts and osteoblasts via membrane-bound ligands and receptors have been proposed to contribute to the coupling between bone formation and resorption [4]

Methods

Results

Conclusion