Abstract
Electromagnetic fields are considered to potentially affect embryonic development, but the mechanism is still unknown. In this study, human embryonic stem cell (hESC) line HUES-17 was applied to explore the mechanism of exposure on embryonic development to pulsed electromagnetic field (PEMF) for 400 pulses at different electric field intensities and the differentiation of HUES-17 cells was observed after PEMF exposure. The expression of alkaline phosphatase (AP), stage-specific embryonic antigen-3 (SSEA-3), SSEA-4 and the mRNA level and protein level of Oct4, Sox2 and Nanog in HUES-17 cells remained unchanged after PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m. Four hundred pulses PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m did not affect the differentiation of HUES-17 cells. The reason why electromagnetic fields affect embryonic development may be due to other mechanisms rather than affecting the differentiation of embryonic stem cells.
Highlights
The development of electromagnetic field application has increased the possibility of human exposure to this potentially harmful factor and raised concern as to its possible ill-health effects
To detect the effects of pulsed electromagnetic field (PEMF) at different electric field intensities on undifferentiated markers in human embryonic stem cell (hESC), the expression of alkaline phosphatase (AP), SSEA3 and SSEA4 in HUES-17 cells was detected after the cells were exposed to 400 pulses PEMF at electric field intensities of 50, 100, 200, 400 or 0 kV/m
The expression of alkaline phosphatase (AP), stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 in HUES-17 cells was detected by AP staining and immunofluorescent staining after the cells were exposed to 400 pulses PEMF at electric field intensities of 50, 100, 200, 400 kV/m or sham-exposed
Summary
The development of electromagnetic field application has increased the possibility of human exposure to this potentially harmful factor and raised concern as to its possible ill-health effects. The hESCs can differentiate into any cell type found in the human body In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm). These cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm) It is for this reason that we are studying the bioeffects of electromagnetic field on hESCs, in order to explore the mechanism of the teratogenic effects of an electromagnetic field and to understand the effects of an electromagnetic field on human heredity and development more directly. One of the reasons for the decrease in success rate as well as an increase in malformations may be due to exposure of the stem cells in early embryonic development to an electromagnetic field during incubation before implantation [10]. Exposure at different electric field intensities in order to explore the mechanism of electromagnetic field on embryonic development
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