Abstract
Background: Proton Pump inhibitors (PPI) are widely used all over the world as therapeutic agents as well as prophylactic agent at different age groups for multiple gastrointestinal disorders etc. it inhibits the hydrogen potassium pump (H/K ATPase Pump). PPI interferes the calcium absorption and thus disturbs acid secretion which leads to decrease the calcium level in the body.
 Objective: The present design is to evaluate the effects of PPI on serum calcium level in rabbit model.
 Study Design: Randomized Control Trial/ Study on Rabbit Model.
 Place and Duration of Study: This experimental study was done at Department of Pharmacology Isra Medical University Hyderabad (November 2020 to March 2021
 Material & Methods: A total 20 healthy male rabbits with weight between 1-2kg were included in this study. While female rabbits, ill rabbits and rabbits with weight less than 1kg were excluded from study. Ten rabbits were given omeprazole sachet of 20mg orally dissolve in water once a day and 10 rabbits were given sachet of esomeprazole of 20mg dissolve in water once a day upto 90 days regularly. At different levels of study blood samples were obtained from vein near ear of rabbits and samples were centrifuged for 10minutes to obtain the serum. Then serum was sent to Isra Diagnostic Laboratory for analysis of serum calcium levels of all samples at different levels of study.
 Results: Serum calcium levels of all rabbits were analyzed at three different levels of study, at zero level before starting of experiment, at level I after completion of one month of experiment and level II after the completion of three months of experiment. The normal serum calcium level in rabbits is 13-15mg/dl.8There was a significant decline in serum calcium level in Group A (Omeprazole Group), while in Group B (Esomeprazole Group) there was no significant relation between decreased serum calcium levels.
 Conclusion: The study concluded that there is significant relation of longterm usage of PPI on serum calcium level.
Highlights
Proton Pump inhibitors (PPI) are widely used all over the world as therapeutic agent as well as prophylactic agent at different age groups for multiple gastrointestinal disorders etc [1].PPI basically is a pro drug[2] which inhibits the hydrogen potassium pump (H/K ATPase Pump) [3]
The gastric H/K-ATPase is composed of two subunits: a catalytic α subunit and a β subunit [5].“The primary structure of the gastric H,K-ATPase α subunit was elucidated in the rat [6] and in the hog [7], rabbit [8], and human [9]
Serum Calcium levels of all rabbits were analyzed at three different levels of study, at zero level before start of experiment, at Level-After the completion of three months of experiment our result shown that there was a significant decline in serum calcium levels in group A which were use omeprazole group of PPI while in group B there was no significant relation between decreased serum calcium levels with esomeprazole group of PPI
Summary
Proton Pump inhibitors (PPI) are widely used all over the world as therapeutic agent as well as prophylactic agent at different age groups for multiple gastrointestinal disorders etc [1].PPI basically is a pro drug[2] which inhibits the hydrogen potassium pump (H/K ATPase Pump) [3]. PPI block the gastric H/K-ATPase, inhibiting gastric acid secretion. The gastric H/K-ATPase is composed of two subunits: a catalytic α subunit and a β subunit [5].“The primary structure of the gastric H,K-ATPase α subunit was elucidated in the rat [6] and in the hog [7], rabbit [8], and human [9] This catalytic subunit consists of 1033 or1034 amino acids with transmembrane segments in all species. PPI interferes the calcium absorption and disturbs acid secretion which leads to decrease the calcium level in the body.
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