Effects of proton pump inhibitors and H2-antagonists on neutrophil oxidative metabolism in patients with gastric and duodenal ulcer bleeding

Abstract

Event Abstract Back to Event Effects of proton pump inhibitors and H2-antagonists on neutrophil oxidative metabolism in patients with gastric and duodenal ulcer bleeding Natalia Obraztsova1*, Oksana Sulaieva1, Stanislav Zharikov1 and Victoria Delii1 1 Donetsk national medical university of M. Gorky, Ukraine Background. Despite the progress in pharmacotherapy and endoscopic management of bleeding 20-30% of patients demonstrate the recurrent bleeding. One of the reasons of it may be inflammation propagation related with dysfunction of neutrophils (Nph). The goal of this research was to estimate the effects of the most common antisecretory drugs as proton pumps inhibitors (PPI) and H2 antagonists (H2A) on Nph oxidative metabolism. Material and methods: Characteristics of bleeding were defined in 124 patients by clinical, laboratory and endoscopic criteria according to Forrest class. In total, 63 patients were treated with PPI, and 61 with H2A. To assess Nph oxidative metabolism, superoxide production was measured using the nitroblue tetrazolium reduction test (NBT) in isolated neutrophils at 1st, 3d and 7th day of therapy. Results. NBT reduction rate was significantly higher in patients with active bleeding (F1a) than that in patients with sustained haemostasis (p<0.05). It has been shown that intensity of superoxide radicals’ production by Nph was higher in patients who were admitted at 12-24 hours than at 3-6 hours after first clinical manifestation of hemorrhage (p<0.05). Administration of PPI was associated with decrease the NBT reduction rate (p<0.01), whereas H2A administration was associated with stimulation of NADPH-oxidase activity (p<0.05) in neutrophils. Conclusion. Administration of PPI and H2A not only affect gastric acid production but also change innate immunity mechanisms since NADPH-oxidase activity of Nph is related with pagocytosis and lifespan. So antisecretory drugs can modulate the acute inflammatory reaction by cells kinetics in ulcer region. Keywords: Neutrophils, bleeding, superoxide, H2-antagonists, PPI Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Obraztsova N, Sulaieva O, Zharikov S and Delii V (2013). Effects of proton pump inhibitors and H2-antagonists on neutrophil oxidative metabolism in patients with gastric and duodenal ulcer bleeding. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00545 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Natalia Obraztsova, Donetsk national medical university of M. Gorky, Donetsk, Ukraine, natalionus@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Natalia Obraztsova Oksana Sulaieva Stanislav Zharikov Victoria Delii Google Natalia Obraztsova Oksana Sulaieva Stanislav Zharikov Victoria Delii Google Scholar Natalia Obraztsova Oksana Sulaieva Stanislav Zharikov Victoria Delii PubMed Natalia Obraztsova Oksana Sulaieva Stanislav Zharikov Victoria Delii Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.