Effects of Prostaglandins E2, F2.ALPHA., and D2 on the Release of Growth Hormone, Prolactin, and Luteinizing Hormone from Cultured Bovine Anterior Pituitary Cells.

Abstract

The objectives of research were to characterize the effects of prostaglandins (PG) on the release of growth hormone (GH), prolactin (PRL), and luteinizing hormone (LH) in cultured bovine anterior pituitary cells. PGE2 significantly increased the media concentrations of GH by 141% (P<0.01) and 27% (P<0.01) at the concentrations of 10-7 and 10-8 M, respectively, compared with controls. The PGE2 significantly increased media concentrations of PRL compared with controls at the concentrations of 10-7 and 10-8 M by 120% (P<0.01) and 22% (P<0.05), respectively. PGE2 also significantly increased LH release at the concentration of 10-7 M by 22% (P<0.05), but not at a concentration of 10-8 M. PGF2α significantly increased the release of LH compared with controls at the concentrations of 10-8 and 10-9 M by 15% (P<0.05) and 19% (P<0.05), respectively, while PGF2α failed to stimulate the release of GH and PRL. In contrast to PGE2 and PGF2α, PGD2 had no effect on the release of GH, PRL, and LH. These results suggest that PGE2 is the most potent prostaglandin among the PGs for the release of GH, PRL, and LH by acting directly on bovine pituitary cells.