Abstract
Granulosa cells were isolated from ovaries (without pre-ovulatory follicles or corpora lutea) of cycling gilts slaughtered at 8 months of age. They were cultured in the presence or absence of exogenous porcine prolactin (1, 10, 1,000, 10,000, or 100,000 pmol/ml medium). The secretion of estrogen, cAMP and oxytocin into the incubation medium was analysed by radioimmunoassay. It was found that prolactin (10, 1,000, 10,000 and 100,000 pmol/ml) inhibited estrogen secretion (p < 0.001) and stimulated cAMP output (p < 0.001, p < 0.001, not significant, and p < 0.01, respectively). Small doses of prolactin (1 and 10 pmol/ml) decreased (p < 0.001, p < 0.05), whilst high doses (1,000, 10,000 and 100,000 pmol/ml) increased (p < 0.001) oxytocin secretion by granulosa cell culture. The data suggest a direct action of prolactin on porcine ovarian cyclic nucleotides, steroid and nonapeptide hormone release.
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