Effects of priming with salicylic acid on germination traits of Dracocephalum moldavica L. under salinity stress

Abstract

In order to evaluate the effects of seed priming with salicylic acid (SA) (0, 2, 10, and 20 mM) to increase salt tolerance (0, 50, 100, 150, and 200 mM NaCl) in medicinal plant Dracocephalum moldavica at seed germination, an experiment was conducted as factorial in a completely randomized design with four replicates. The assessed parameters included germination percentage and rate, length, dry weight, lipid peroxidation, electrolyte leakage, and the activity of catalase, ascorbate peroxidase, and guaiacol peroxidase of the seedlings. Results revealed the values of germination indices significantly decreased with increasing salinity levels. Nevertheless, seed priming with SA (2 mM) significantly mitigated the adverse effects of salinity in D. moldavica. Under salinity and at this level of SA priming, seed germination percentage increased by 24% and 75% at 100 and 150 mM compared to exclusive salt stress. Also, germination rate augmented by SA priming up to 9.2% at 100 mM and 2 folds at 150 mM NaCl. The values of weight (+ 9.1% at 100 mM and +8 folds at 150 mM) and length (+12.5% at 100 mM and + 15.1% at 150 mM NaCl) of seedlings significantlyincreased by SA priming compared to the exclusively salt-stressed ones. SA priming increased antioxidant enzymes activities while it decreased the level of lipid peroxidation and ion leakage in the seedlings of D. moldavica. As a conclusion, SA improved seed performance in D. moldavica under salt stress by reduction of detrimental effects of oxidative stress.