Effects of Pretreatment With Yisheng Injection on Renal Warm Ischemia/Reperfusion Injury in Mice

Abstract

Introduction Due to the organ supply shortage, the donor pool has been expanded to include non–heart-beating donors, where renal warm ischemia/reperfusion (I/R) injury is inevitable. This study was undertaken to determine whether Yisheng injection (YM) could attenuate renal warm I/R injury in mice. Materials and Methods Male C57BL/6 mice were divided into sham, ischemic, and YM-treated groups using 50 minutes of left kidney ischemia. Mice were humanely killed at 4 or 24 hours postreperfusion. We assayed the effects of YM on liver functional injury, neutrophil recruitment, and proinflammatory mediators after renal I/R injury. Results Renal I/R produced dramatic injuries in mouse kidneys. Administration of YM reduced liver function (urea nitrogen of untreated vs treated, 4.7 ± 0.6 vs 26.6 ± 1.5 mmol/L; P < .01) and histological injury (histological scores of untreated vs treated, 4.12 ± 0.14 vs 0.98 ± 0.07; P < .01). YM at doses of 5, 15, or 25 mg/kg reduced the serum levels of tumor necrosis factor-alpha (TNF-α) by about 32.9%, 55.1%, and 74.5%, respectively. Moreover, YM also suppressed the increase in messenger RNA (mRNA) and protein expressions of TNF-α and intercellular adhesion molecule-1 (ICAM-1), as well as abrogated neutrophil recruitment in a dose-dependent manner. Conclusion YM protects murine kidneys from warm I/R injury, probably via decreasing functional injury, reducing neutrophil infiltration, and suppressing the overexpression of proinflammatory mediators and adhesion molecules.