Effects of pretreating in vitro matured of native Thai cattle oocyte with docetaxel before vitrification on their viability

Abstract

The present study was to investigate the efficacy of docetaxel (DT) at reducing microtubule damage during vitrification and maintaining the developmental competence of vitrified-warmed oocytes in native Thai cattle. Native Thai cattle cow oocyte from ultrasound-guided transvaginal follicular aspiration (UTFA) were subjected to in vitro maturation (IVM). Then, the IVM oocytes were pretreating with DT in various concentration (0.0, 1.0, 2.0 and 3.0 µM) and subjected to cryopreservation by Cryotop vitrification method. The survival rate of vitrified-warmed oocyte, cleavage and blastocyst formation rate after in vitro fertilization (IVF), and blastocyst nucleic cell numbers were evaluated in this study. Pretreatment of IVM native Thai cattle oocytes with 0.05 µM DT before vitrification resulted in significantly higher (P < 0.05) rates of oocyte survival and cleavage after IVF, and subsequent blastocyst formation on Days 7-8, hatching and hatched on Days 8-9, compared with oocytes pretreated with 0.5 and 1.0 µM DT before vitrification or those vitrified and the control group. Pretreatment of IVM native Thai cattle oocytes with 0.05, 0.5 and 1.0 µM DT before vitrification without side effects on blastocyst nucleic cell numbers. In summary, pretreatment of IVM native Thai cattle oocytes with 0.05 μM DT before vitrification improved survival of vitrified-warmed oocytes, fertilization and developmental competence.