Abstract
To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocyte meiotic maturation percentage, mitochondrial distribution and intracellular glutathione (GSH) level were evaluated. The results showed no surviving oocytes could be collected by 2h, 6h, and 12 h after carcass preservation at 37°C, 25°C and 4°C, respectively. The number of collected GV oocytes in the ovary deceased as the preservation time lasted at the same temperature. Meanwhile at the same point in time, the ratio of germinal vesicle breakdown (GVBD) and the first polar body emission (PBE) gradually reduced as preservation temperature increased. In addition, the percentage of abnormal mitochondrial distribution in the preserved oocytes was obviously higher than that in the control oocytes, while GSH level was not altered in collected oocytes. Unexpectedly, neither chromosome arrangement nor spindle organization was affected as long as the oocytes from preserved carcasses could complete maturation. These data are helpful for proper use of ovary oocytes from postmortem carcass of valuable individuals.
Highlights
Experimental animals play an important role in scientific research
To study the oocyte meiotic maturation ablity, we counted the percentages of germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) which were the most obvious indicators of oocyte maturation
ICR mice were killed and carcasses were kept at a room temperature (25uC) for different hours, Fig. 1Aa showed that the number of collected germinal vesicle (GV) oocytes decreased with preservation time
Summary
Experimental animals play an important role in scientific research. Most of the oocytes used for studies are from slaughtered animals. Ovaries are usually removed immediately after the death of animals, but sometimes it is not convenient to conduct immediate experiments and oocyte may be taken from animals that die for a period of time. The length of time after the death of animals may be one of the important factors affecting oocyte quality. Schroeder et al compared the capacity of oocytes matured in vitro following isolation at the GV stage from freshly killed mice with that of oocytes from the carcasses of mice killed more than 3 hours earlier. They found that maturation did not differ between oocytes from freshly killed mice and those collected from mice killed 6 hours earlier [1]. It is necessary to clarify the postmortem time effect on ovary oocyte vitality
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