Effects of Polygonati Rhizoma Extracts on the Collagenase Activity and Procollagen Synthesis in Hs68 Human Fibroblasts and Tyrosinase Activity

Abstract

Objectives : This study was designed to investigate the collagen metabolism and tyrosinase activity of Polygonati Rhizoma extracts (PR). It's effects are to tonify spleen qi and augment the spleen yin. It enrichs the yin and moisten the lung. Methods : The effect of PR on type I procollagen production and collagenase (matrix metalloproteinase-1, henceforth referred as MMP-1) activity in human normal fibroblasts Hs68 after ultraviolet B (UVB, 312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of PR was measured. Results : There were no cytotoxicity at concentrations of 10, 30, <TEX>$100{\mu}g/ml$</TEX>. The reduced type I procollagen production was recovered by PR in UVB damaged Hs68 cells at a concentration of <TEX>$100{\mu}g/ml$</TEX> (<TEX>$16.2{\pm}0.0$</TEX> ng/ml) from control group (<TEX>$13.9{\pm}0.5$</TEX> ng/ml). However there was no statistical significance. PR reduced The increased MMP-1 activity after UVB damage at concentrations of <TEX>$10{\mu}g/ml$</TEX>, <TEX>$30{\mu}g/ml$</TEX>, and <TEX>$100{\mu}g/ml$</TEX> in a dose dependent manner (<TEX>$42.2{\pm}20.5%$</TEX>, <TEX>$44.8{\pm}8.5%$</TEX>, and <TEX>$22.0{\pm}5.8%$</TEX>). PR <TEX>$100{\mu}g/ml$</TEX> treatment showed the statistical significace (p < 0.05). PR significantly reduced the tyrosinase activity at a concentration of 10 mg/ml (<TEX>$32.0{\pm}12.8%$</TEX>, p < 0.05). However, the L-DOPA oxidation was not changed. Conclusion : PR showed the anti-wrinkle effects and whitening effects in vitro. Although more researches are needed to validate the efficacy, these results suggest that PR may have potential as an anti-aging ingredient in cosmetic herb markets.