Abstract
Objective: The objective of this study was to analyze the intrafibrillar remineralization process and the size of hydroxyapatite crystals formed.Methods: The samples were divided into four groups. The control group was immersed in a demineralization solution, whereas the remaining threetreatment groups were immersed in a remineralization solution containing polyaspartic acid for 3, 7, or 14 days. The effect of polyaspartic acid onintrafibrillar remineralization during the polymer-induced liquid-precursor (PILP) process was evaluated using transmission electron microscopy,and the size of hydroxyapatite crystals was examined through X-ray diffraction.Results: Significant differences were detected in the intrafibrillar remineralization between the treatment groups (subjected to remineralization for3, 7, and 14 days) and the demineralized dentin control group. However, no significant differences were detected in the size of hydroxyapatite crystalsbetween the control and the treatment groups.Conclusion: Our data suggest that polyaspartic acid enables intrafibrillar remineralization during the PILP process.
Highlights
Dentin caries is classified into infected dentin and affected dentin [1,2]
The transmission electron microscopy (TEM) was performed to visualize the occurrence of intrafibrillar remineralization characterized by mineral deposits in the intrafibrillar collagen
The X-ray diffraction (XRD) examination was carried out to evaluate the size of hydroxyapatite crystals [16]
Summary
Dentin caries is classified into infected dentin and affected dentin [1,2]. Infected dentin is the outermost layer of caries consisting of a mineral matrix and hyrdroxyapatite crystals which are destroyed by a bacterial infection and its byproducts. The damage is irreversible and inhibits remineralization. By contrast, affected dentine is the inner layer that is partially demineralized, but the damage is still reversible. The affected dentine zone can undergo demineralization as it contains healthy collagen fibrils surrounded by hydroxyapatite crystals and collagen crosslinks [2,3]. Dentin remineralization can occur due to the deposition of minerals between collagen fibers [4]
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