Effects of poly(amidoamine) dendrimers on the structure and function of key blood components

Abstract

Poly(amidoamine) dendrimers have a variety of promising biomedical applications; however, the biological safety of the dendrimers has not been well clarified. This study focuses on the effects of poly(amidoamine) dendrimers (G3, G4, G5, G5–OH) on the structure and function of key blood components, in order to elucidate the impacts of the dendrimers on the aggregation, morphology, lysis of human red blood cells, structural and conformational change as well as polymerization of fibrinogen, and the coagulation of blood tissue. The poly(amidoamine) dendrimers caused aggregation, morphological changes, and lysis of red blood cells, depending on the dendrimer concentration, generation, and surface functional groups. All the dendrimers induced fibrinogen structural and conformational changes, but only cationic dendrimers impaired fibrinogen polymerization ability at high concentrations. In addition, the cationic dendrimers inhibited the activity of clotting factors and fibrinogen in the coagulation of whole blood. Therefore, the surface functional groups, generation, and concentration of the dendrimers play important roles in affecting the structure and function of key blood components. These results provide a critical theoretical basis for the molecular design and clinical application of the poly(amidoamine) dendrimers.