Abstract
Background: Particulate matter (PM) is an air pollutant that can damage human skin. Antioxidants have been tested to improve skin inflammation induced by PM. Objective: We investigated the effects of punicalagin on PM-induced inflammation on cultured human sebocytes, outer root sheath (ORS) cells, and Cutibacterium (C.) acnes-pretreated mice. Methods: Sebocytes and ORS cells were cultured and treated with 5-μM punicalagin and 100-μg/mL PM10 for 24 h. On C. acnes-pretreated mice, 5-μM punicalagin and 100-μg/mL PM10 were applied. Cell viability was measured for MTT assay. Real-time PCR and measurement of reactive oxygen species as well as sebum production were conducted to analyze its effects. Results: Upregulation of the gene expression of inflammatory cytokines, matrix metalloproteinase, aryl hydrocarbon receptor, and NF-kB by PM10 in cultured sebocytes and ORS cells and the increase in the production of reactive oxygen species (ROS) by PM10 in cultured sebocytes were inhibited by punicalagin. Moreover, punicalagin decreased inflammatory cytokines, matrix metalloproteinase, and sebum production in C. acnes-pretreated mice. Conclusions: Punicalagin effectively reduces the expression of inflammatory biomarkers and the production of sebum in cultured sebocytes, ORS cells, and C. acnes-pretreated mice.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
