Abstract
BackgroundHuman menstrual blood-derived stromal cells (MenSCs) are highly proliferative and show multiple differentiation capacity. The convenience and non-invasiveness make MenSC a novel cell source for regenerative medicine applications. Platelet-rich plasma (PRP) contains abundant growth factors which are beneficial to wound healing. However, the influence of PRP on MenSCs remains elusive. Here, we evaluated the role of PRP in MenSCs proliferation and assessed the effects of PRP on endometrial receptivity regulation in vitro.MethodsMenSCs cultured with 10% activated PRP were compared with those cultured with 10% fetal bovine serum (FBS). Differences in cell proliferation, differentiation, and endometrial receptivity-related gene expression were evaluated.ResultsNotably, 10% activated PRP significantly promoted MenSCs proliferation and adipogenic/osteogenic differentiation while suppressing apoptosis. Expression of the mesenchymal stem cells (MSCs) marker CD105 and the perivascular markers SUSD2 and CD146 were elevated after PRP treatment. Moreover, short-term PRP stimulation activated the phosphorylation of Akt and signal transducer and activator of transcription 3 (STAT3) pathways, upregulated expression of FoxO1, LIF, and IL1-β, and downregulated IL-6.ConclusionsIn summary, PRP could promote MenSC proliferation, markedly accelerate cell stemness, and evaluate MenSC functions by enhancing the expression of angiogenesis and endometrial receptivity markers, suggesting its potential use as a promising supplement for MenSCs in endometrial regenerative medicine. Our results provide a theoretical basis for the clinical application of co-transplantation of PRP combined with MenSCs.
Highlights
Human menstrual blood-derived stromal cells (MenSCs) are highly proliferative and show multiple differentiation capacity
Platelet-rich plasma (PRP) promoted MenSCs proliferation while protecting cells from apoptosis First, we examined the effects of PRP on MenSCs proliferation using cell counting kit-8 (CCK8) assays (n = 3)
The positive rate was much higher after 24 h of culture than after 48 h of culture in both groups. These results indicated that PRP acted positively on DNA replication in MenSCs, suggesting that PRP may elicit stronger effects after a shorter incubation
Summary
Human menstrual blood-derived stromal cells (MenSCs) are highly proliferative and show multiple differentiation capacity. Menstrual blood-derived stromal cells (MenSCs) are isolated human endometrial stem cells (hENSCs) obtained during menstrual shedding [1]. The perivascular markers CD146, platelet-derived growth factor receptor (PDGFR)-β, and sushi domaincontaining 2 (SUSD2) can be used as specific markers for isolation of MenSCs directly from the endometrial shedding mixture by flow cytometry [3]. Neither tumor growth nor toxicity was detected after MenSCs treatment in animal models. We transferred autologous MenSCs to seven infertile women who were diagnosed with severe Asherman’s syndrome and demonstrated that the endometrial thickness of all patients was significantly increased, including in one patient who had an ongoing pregnancy. Our results suggest that MenSCs transplantation may be a promising option for endometrial regeneration and improvement of endometrial receptivity improvement [10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
