Effects of platelet-activating factor, thromboxane A 2 and leukotriene D 4 on isolated perfused rat liver

Abstract

Vasoconstrictive lipid mediators, thromboxane A 2 (TxA 2), platelet-activating factor (PAF) and leukotriene D 4 (LTD 4) have been implicated as mediators of liver diseases. There are species differences in the primary site of hepatic vasoconstriction in response to these mediators. We determined the effects of a TxA 2 analogue (U-46619), PAF and LTD 4 on the vascular resistance distribution, weight and oxygen consumption of isolated rat livers portally perfused with blood. The sinusoidal pressure was measured by the double occlusion pressure ( P do), and was used to determine the pre- ( R pre) and post-sinusoidal ( R post) resistances. All these three mediators increased the hepatic total vascular resistance ( R t). The responsiveness to PAF was 100 times greater than that to U-46619 or LTD 4. Both of PAF and U-46619 predominantly increased R pre over R post. At the comparable increased R t levels, U-46619 more preferentially increased R pre than PAF. In contrast, LTD 4 increased both the R pre and R post to similar extent. U-46619 caused liver weight loss, while high concentrations of either LTD 4 or PAF produced liver weight gain, which was caused by substantial post-sinusoidal constriction and increased P do. PAF and U-46619 decreased hepatic oxygen consumption while LTD 4 induced biphasic change of an initial transient decrease followed by an increase. In conclusion, PAF is the most potent vasoconstrictor of rat hepatic vessels among these three mediators. Both TxA 2 and PAF constrict the pre-sinusoidal veins predominantly. TxA 2 more preferentially constricts the pre-sinusoids than PAF, resulting in liver weight loss. However LTD 4 constricts both the pre- and post-sinusoidal veins similarly. High concentrations of LTD 4 and PAF cause liver weight gain by substantial post-sinusoidal constriction. PAF and TxA 2 decrease hepatic oxygen consumption, whereas LTD 4 causes a biphasic change of it.