Effects of planting year of alfalfa on rhizosphere bacterial structure and function

Abstract

In many areas across the world, alfalfa is still cultivated in the same place over many years, leading to gradually deplete the soil nutrients and increase plant disease and consequent decreases in the yield and quality. Agricultural management practices have been proved to affect largely soil microbial community structure and functions in many reports, but it remains unclear how planting year effects extend to the rhizosphere under a long-term cultivation practices of alfalfa. Exploring such impacts is of great importance to understand and develop plant-microorganism interactions for agricultural sustainability. A long-term field experiment was set up at Linze research station of Lanzhou University in Gansu province, where alfalfa was continuously planted for four years (Y4) and six years (Y6). Rhizosphere soils from two different planting years were collected to examine differences in the bacterial diversity, composition and co-occurrence patterns using 16S rRNA gene sequencing technologies as well as in functional profiles involved in nitrogen cycling predicted by FAPROTAX. We found that soil nutrients including soil organic carbon (SOC), AP (available phosphorus) and nitrate nitrogen (NO3−-N) differed significantly between planting years. Bacterial taxonomic diversity, composition and the relative abundance of functional groups involved in nitrogen cycling were significantly affected by planting years, sampling time and their interactions. Co-occurrence network analysis showed that keystone taxa in both planting years were affiliated to Proteobacteria and bacteria taxa had less complexity and more negative correlations in Y6 than in Y4. NO3−-N was a major factor affecting bacterial community diversity and composition across planting years. Together, our findings suggest that planting years of alfalfa can lead to large variations in soil nutrients and rhizosphere microbial community patterns. These results implies that in agricultural management practices, planting year effects can even extend to microorganisms in association with plant roots, which may affect crop growth given close linkages between rhizosphere processes and plant nutrition availability.