Effects of plant functional diversity induced by grazing and soil properties on above- and belowground biomass in a semiarid grassland

Abstract

The trait-based plant functional diversity is associated with ecosystem functioning. However, few studies show the effects of plant functional diversity induced by grazing disturbance and environmental changes on aboveground plant biomass (AGB) and belowground root biomass (BGB) in semiarid grasslands. We examined the effects of long-term grazing on plant functional diversity across four grassland types (meadow, steppe, scattered tree grassland and sandy grassland) in Horqin grassland, Northern China. The structural equation model (SEM) was used to evaluate the direct or indirect effects of long-term grazing, soil properties and functional diversity reflected by the single-trait (community-weighted mean) and multi-trait (functional dispersion, FDis) on AGB and BGB across four grassland types. We found that long-term grazing significantly decreased plant height and FDis, while the responses of leaf traits to grazing differed among four grassland types. The correlation analyses showed that AGB and BGB were negatively associated with grazing and positively associated with plant height, FDis, soil carbon (C) and nitrogen (N). The SEM results indicated that AGB was directly affected by grazing, soil N, plant height and perennial richness, and BGB was directly affected by grazing, soil N, soil water content and elevation. Grazing and soil N also indirectly affected AGB through their effects on plant height and FDis. Effect of plant height on aboveground plant biomass was direct rather than indirect, while FDis acted indirectly through its effect on perennial richness, thereby lending more support to the mass ratio hypothesis. Our results clearly highlight the critical role of plant functional diversity induced by grazing and soil properties in affecting AGB in a semiarid grassland ecosystem. So, we recommend considering the linkages of plant functional diversity with ecosystem function in assessing the effects of grazing and soil changes on grassland ecosystems.