Effects of plant diversity on microbial biomass and community metabolic profiles in a full-scale constructed wetland

Abstract

There has been less understanding of relations of microbial community patterns with plant diversity in constructed wetlands. We conducted a single full-scale subsurface vertical flow constructed wetland (SVFCW, 1000 m 2) study focusing on domestic wastewater processing. This study measured the size and structure of microbial community using fumigation extraction and BIOLOG Ecoplate™ techniques, to examine the effects of macrophyte diversity on microbial communities that are critical in treatment efficiency of constructed wetlands. We also determined the relationship of plant diversity (species richness) with its biomass production under disturbance of the same wastewater supply. Linear regression analysis showed that plant biomass production strongly correlated with plant species richness ( R = 0.407, P < 0.001). Increase in plant species richness increased microbial biomass carbon and nitrogen ( R = 0.494, P < 0.001; R = 0.465, P < 0.001) and utilization of amino acids on Ecoplates ( R = 0.235, P = 0.03), but limited the utilization of amine/amides ( R = −0.338, P = 0.013). Principal components analysis (PCA) showed that the diversity and community-level physiological profiles (CLPP) of microbial community at 168 h of incubation strongly depended on the presence or absence of plant species in the SVFCW system, but not on the species richness. This is the first step toward understanding relations of plant diversity with soil microbial community patterns in constructed wetlands, but the effect of species diversity on microbial community should be further studied.