Abstract
Genomic DNA was extracted from excised gallbladder mucosa, obtained shortly after a single treatment piezoelectric lithotripsy. Control samples were obtained from age and sex-matched patients who had received no lithotripsy. No DNA fragmentation was demonstrable on agarose gel electrophoresis. Suspensions of cultured lymphoblasts were subjected to different shock wave doses and the percentage of cells surviving the treatment was assessed immediately and the rate of cell death monitored over the following 7 h. The proportion of cells surviving the treatment decreased with increasing shock wave doses, but subsequent rate of cell death was no different from controls. DNA, extracted from the intact surviving cells, again showed no evidence of fragmentation on electrophoresis, but the DNA extracted from the supernatant showed extensive fragmentation.
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