Effects of Phytic Acid-Degrading Bacteria on Mineral Element Content in Mice.

Diao Zhou,Ying Zhao,Jia Yin,Qiuyun Huang,Hengjia Ni,Cang Chen,Jing Li,Vinothkannan Ravichandran,Leli Wang

doi:10.3389/fmicb.2021.753195

Abstract

Trace minerals are extremely important for balanced nutrition, growth, and development in animals and humans. Phytic acid chelation promotes the use of probiotics in nutrition. The phytic acid-degrading strain Lactococcus lactis psm16 was obtained from swine milk by enrichment culture and direct plate methods. In this study, we evaluated the effect of the strain psm16 on mineral element content in a mouse model. Mice were divided into four groups: basal diet, 1% phytic acid, 1% phytic acid + psm16, 1% phytic acid + 500 U/kg commercial phytase. Concentrations of acetic acid, propionic acid, butyric acid, and total short-chain fatty acids were significantly increased in the strain psm16 group compared to the phytic acid group. The concentrations of copper (p = 0.021) and zinc (p = 0.017) in liver, calcium (p = 0.000), manganese (p = 0.000), and zinc (p = 0.000) in plasma and manganese (p = 0.010) and zinc (p = 0.022) in kidney were significantly increased in psm16 group, while copper (p = 0.007) and magnesium (p = 0.001) were significantly reduced. In conclusion, the addition of phytic acid-degrading bacteria psm16 into a diet including phytic acid can affect the content of trace elements in the liver, kidney, and plasma of mice, counteracting the harmful effects of phytic acid.

Highlights

Trace elements play an important role in the growth, development, and metabolism of animals and humans
To verify whether strain psm16 affects the content of mineral elements in mice, we studied the calcium, magnesium, copper, zinc, iron, phosphorus, and manganese levels in the liver, kidney, and plasma
In mice gavaged with strain psm16, a higher content of mineral elements was found in the organs of mice

Summary

Introduction

Trace elements play an important role in the growth, development, and metabolism of animals and humans. Significant fluctuations in their levels can lead to metabolic disorders and immune dysfunction (Cannas et al, 2020). Phytic acid is present in many plant organs, accounting for 1–5% of most seeds, roots, and stems of grains and vegetables (Wang et al, 2013). Numerous animals feed on fiber-rich grains while the daily intake of humans is influenced by culture and society (Wang and Guo, 2021). Phytic acid is a sugar phosphate with six strong negative charges and has commonly been reported as an anti-nutritional factor in humans and animals due to its strong

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