Effects of physiological integration on photosynthetic efficiency of Trifolium repens in response to heterogeneous UV-B radiation

Abstract

Several studies have found the photosynthetic integration in clonal plants to response to resource heterogeneity, while little is known how it responses to heterogeneity of UV-B radiation. In this study, the effects of heterogeneous UV-B radiation (280-315 nm) on gas exchange and chlorophyll fluorescence of a clonal plant Trifolium repens were evaluated. Pairs of connected and severed ramets of the stoloniferous herb T. repens were grown under the homogeneity (both of ramets received only natural background radiation, ca. 0.6 kJ m-2 d-1) and heterogeneity of UV-B radiation (one of the ramet received only natural background radiation and the other was exposed to supplemental UV-B radiation, 2.54 kJ m-2 d-1) for seven days. Stomatal conductance (g s), intercellular CO2 concentration (C i) and transpiration rate (E) showed no significant differences in connected and severed ramets under homogenous and heterogeneous UV-B radiation, however, net photosynthetic rate (P N) and maximum photosynthetic rate (P max) of ramets suffered from supplemental increased UV-B radiation and that of its connected sister ramet decreased significantly. Moreover, additive UV-B radiation resulted in a notable decrease of the minimal fluorescence of dark-adapted state (Fo), the electron transport rate (ETR) and photochemical quenching coefficient (qP) and an increase of nonphotochemical quenching (NPQ) under supplemental UV-B radiation, while physiological connection reverse the results. In all, UV-B stressed ramets could benefit from unstressed ramets by physiological integration in photosynthetic efficiency, and clonal plants are able to optimize the efficiency to maintain their presence in less favourable sites.