Abstract
Free, esterified and glycosylated sterols of pea leaves were investigated during different stages of ageing. Samples for sterol analysis were collected both from leaves of different maturity of plants with 12 leaves and from newly unfolded leaves of plants at juvenile and mature stage. Free sterols were the most abundant form. The total level of sterols decreased with increasing physiological age, mainly due to a decline in the levels of stigmasterol and an unidentified sterol, while sitosterol remained constant. The total content of sterols was highest in the newly unfolded leaves from the juvenile plants. The unidentified sterol decreased from juvenile to mature stage, while stigmasterol and sitosterol remained constant. The results indicate that the membrane sterol composition reflects the developmental level that is prevailing. Stigmasterol might have a regulatory function in physiological ageing.
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