Effects of Photoperiod on Salmon GnRH mRNA Levels in Brain of Castrated Underyearling Precocious Male Masu Salmon

Abstract

Previous studies have suggested that activation of salmon gonadotropin-releasing hormone (sGnRH)-producing neurons is induced by the combined effects of photoperiod and steroid hormones in underyearling males of the masu salmon, Oncorhynchus masou. The present study further assesses the effects of photoperiod and steroid hormones on sGnRH synthetic activity and examines the changes in sGnRH mRNA levels in the brains of castrated underyearling precocious male masu salmon by manipulating the photoperiod for 60 days from August through October. In castrated males in which plasma testosterone levels decreased to low levels, sGnRH mRNA levels in the preoptic area (POA) increased under a short photoperiod (8L-16D), whereas they remained at low levels under a long photoperiod (16L-8D) for a 2-month duration. In sham-operated males, sGnRH mRNA levels in the ventral telencephalon and those in the POA increased in October with testicular maturation even under a long photoperiod with a delay of 1 month compared with the short photoperiod group. These results suggest that preoptic sGnRH-producing neurons receive short photoperiodic signals and that either short photoperiod or steroid hormone secretion is required for the activation of sGnRH synthesis in underyearling precocious male masu salmon.