Effects of photodynamic therapy, 2% chlorhexidine, triantibiotic mixture, propolis and ozone on root canals experimentally infected with Enterococcus faecalis: an in vitro study.

Abstract

The aim of this study was to evaluate the antibacterial effect of photodynamic therapy (PDT), 2% chlorhexidine (CHX). The teeth were contaminated with 0.1mL Enterococcus faecalis (3×108cell/mL) and randomized into eight treatment groups: Group 1 (2.5% NaOCl); Group 2 (PDT); Group 3 (2% CHX); Group 4 (TAM); Group 5 (propolis), Group 6 (ozone), Group 7 (positive control) and Group 8 (negative control). Following treatment, the canal content was sampled. The samples were cultured in triplicate on blood agar plates to determine the number of colony forming units (CFU)/mL. The teeth were analyzed by SEM to determine the percentage of area with contamination and debris. The group with the lowest CFU/mL count was the ozone group, which obtained similar values to the PDT group. SEM images showed that 2.5% NaOCL group showed the lowest percentage of contaminated area. Applications of PDT, 2% CHX, TAM, propolis and ozone showed antibacterial potential similar to 2.5% NaOCL.