Effects of phosphorus concentration on the growth and enzyme production of Phanerochaete chrysosporium

Abstract

The effects of different phosphorus concentrations in culture media on the growth and enzyme production of Phanerochaete chrysosporium was investigated at a glucose concentration of 10gL−1. The results showed that the optimal KH2PO4 concentration was 2.0gL−1. Optimal phosphorus content not only supported robust growth of P. chrysosporium, but also helped produce higher yields of manganese-dependent peroxidase (MnP) (324.9UL−1). In addition, the results revealed that a relationship between the consumption of total phosphorus (TP) and fungal growth and enzyme production existed in P. chrysosporium cultures. Over a range of 0–0.5gL−1 KH2PO4 concentration in the medium, the biomass and MnP activity increased in proportion to phosphorus concentration. When the KH2PO4 concentration reached 0.5gL−1, it was generally found that the increase in biomass gradually slowed down, while MnP production decreased greatly with an increase in phosphorus concentration.