Effects of phosphorus availability and phosphorus utilization behavior of Microcystis aeruginosa on its adaptation capability to ultraviolet radiation

Abstract

Phosphorus (P) plays a critical role in eutrophication and algal growth; therefore, improving our understanding of the impact of P is essential to control harmful algal blooms. In this study, Microcystis aeruginosa was treated with 5-h ambient irradiation in the medium with different dissolved inorganic P (DIP) concentrations, DIP-free, moderate-DIP, and high-DIP, to explore its growth and other physiological responses. Compared to photosynthetically active radiation (PAR), UV-A (320–400 nm) and UV-B (280–320 nm) radiation had inhibitive effects on the photosynthesis and growth of M. aeruginosa, while high P availability could alleviate or eliminate the negative effects of UV radiation. The photosynthetic parameters had a minimum reduction and quickly recovered after re-inoculation under high-DIP conditions. Confirmed by SEM, photosynthetic pigments, the generation of reactive oxygen species (ROS), superoxide dismutase (SOD) activity and other methods, ambient UV radiation exerted oxidative stresses rather than direct lethal effects on M. aeruginosa. Photosynthetic parameters indicated that algal UV-adaptation processes could include decreasing photo-induced damages and increasing self-repair efficiency. The P acquired by M. aeruginosa cells can have two function, which included alleviating UV-induced negative effects and sustaining algal growth. Consequently, UV-adaptation processes of M. aeruginosa resulted in an elevated demand for DIP, which resulted to increased P uptake rates and cellular P quota under moderate and high-DIP conditions. Therefore, the production of carotenoid and phycocyanin, and SOD activity increased under UV stress, leading to a better adaptation capability of M. aeruginosa and decreased negative effects of UV radiation on its growth. Overall, our findings demonstrated the significant interactive effects of P enrichment and irradiation on typical cyanobacteria, and the strong adaptation capability of M. aeruginosa in the eutrophic UV-radiated waters.