Abstract
Caffeine intake is strongly linked to lipid metabolism. We previously reported the age-dependent physiological effects of caffeine intake in a Caenorhabditis elegans model. Since nutritional status can actively influence metabolism and overall health, in this study, we evaluated the effect of caffeine intake on lipid metabolism in adult-stage C. elegans. We found that, in C. elegans, fat storage and the level of phosphoethanolamine (PE) were significantly reduced with caffeine intake. In addition, mitochondrial activity decreased and mitochondrial morphology was disrupted, and the expression of oxidative stress response genes, hsp-6, gst-4, and daf-16, was induced by caffeine intake. Furthermore, the level of an energy metabolism sensor, phospho-AMP-activated protein kinase, was increased, whereas the expression of the sterol regulatory element binding protein gene and its target stearoyl-CoA desaturase genes, fat-5, -6, and -7, was decreased with caffeine intake. These findings suggest that caffeine intake causes mitochondrial dysfunction and reduces lipogenesis. Interestingly, these changes induced by caffeine intake were partially alleviated by PE supplementation, suggesting that the reduction in mitochondrial activity and lipogenesis is in part because of the low PE level, and proper dietary supplementation can improve organelle integrity.
Highlights
Caffeine is the most popular drug consumed worldwide; approximately 80% of the world’s population consumes caffeinated foods every day [1,2]
We investigated the control of lipid metabolism by 10 mM of caffeine intake in adult-stage C. elegans and its association with mitochondrial activity
Previous studies have suggested that caffeine intake affects lipid metabolism [7,24,25]
Summary
Caffeine is the most popular drug consumed worldwide; approximately 80% of the world’s population consumes caffeinated foods every day [1,2]. The increased cAMP response is short-lived because it is rapidly degraded by phosphodiesterase (PDE). Caffeine has been associated with loss in body weight and increased energy expenditure in humans and animal models [4,5,6,7]. These studies indicate the strong relationship between caffeine intake and lipid metabolism. It is largely unknown how caffeine intake modulates various physiological processes in animals including lipid metabolism at the molecular level
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
