Effects of phosphate, surfactants and glucose on phytase production and hydrolysis of phytic acid in canola meal by Aspergillus ficuum during solid-state fermentation

Abstract

It was noticed that phosphate concentration influenced phytase production in canola meal during the growth of Aspergillus ficuum in a solid-state fermentation process. Lower concentrations of this compound favoured the production of the enzyme. Compared with the control, Tween-80 and sodium oleate increased the rates of phytase production and hydrolysis of phytic acid, while Triton X-100 had a negative effect on these processes. Glucose concentrations of up to 5·2% increased the biomass growth, production of phytase and the rate of phytic acid hydrolysis, while systems with 9·8 and 17·8% of glucose had an adverse effect on these processes. Models were given which fit the experimental data of biomass production and the hydrolysis of phytic acid reasonably well. The suggested model for the production of enzyme should be improved for the systems with higher glucose concentrations.