Effects of Phaseolus vulgaris Extract on Lipolytic Activity and Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes: A Strategy to Prevent Obesity.

Felipe Castillo,Rodrigo Moore-Carrasco,Daniel R González

doi:10.1155/2019/5093654

Abstract

Background Overweight and obesity are defined as abnormal or excessive fat accumulation that may be harmful for health. A global trend in this area is the search for natural compounds that have a proven beneficial effect and no clinical complications. Phaseolus vulgaris (bean) is a vegetable highly consumed worldwide. One of its effects, the most reported, is weight reduction in overweight individuals. Objective The objective of this study was to investigate the antiobesity activity of this legume in mature 3T3-L1 adipocytes and in rat white adipose tissue in an ex vivo model. Design Mature adipocytes 3T3-L1 and rat adipose tissue were treated with bean extracts. We quantified lipolysis in mature 3T3-L1 adipocytes and in rat white adipose tissue in an ex vivo model. Results In an ex vivo assay with adipose tissue, methanolic and aqueous green bean extracts increased glycerol release to the medium compared to control (p < 0.05 and p < 0.001 respectively). Treatment of 3T3-L1 adipocytes with green bean extracts (800 and 1000 µg/mL) increased glycerol release significantly (p < 0.0001). Extracts at concentrations between 500 and 1000 µg/mL reduced intracellular triglyceride accumulation by 34.4% and 47.1% compared to control (p < 0.0001). Discussion Our results propose that bioactive compounds of green beans exert a direct mechanism on adipocytes through lipolysis. Conclusion We have identified a novel capacity of bean extracts related to lipolytic activity both in vitro and ex vivo, resulting in a powerful lipolytic effect. Moreover, we also found that bean extracts has an antiadipogenic effect during the differentiation of 3T3-L1 preadipocytes. These results suggest that bean is a good candidate for the development of functional ingredients that can help reduce the high rates of death from cardiovascular diseases associated with obesity.

Highlights

Obesity is defined as having a body mass index greater than 30 kg/m2 and has been recognized as a global epidemic with a myriad of detrimental health effects [1]
To assess the ability of bean extracts to stimulate lipolysis under physiological conditions, a series of ex vivo assays with rat adipose tissue was performed. e dorsal adipose tissue was removed from SD rats; this was washed with saline and incubated in the presence of different extracts at 1 mg/ml concentration
Our aim was to evaluate the ability of bean extracts to induce direct lipolysis in adipose tissue. is effect has not been reported for these beans, and the results clearly show that the bean extracts significantly stimulated lipolysis in rat adipose tissue ex vivo

Summary

Introduction

Obesity is defined as having a body mass index greater than 30 kg/m2 and has been recognized as a global epidemic with a myriad of detrimental health effects [1]. E objective of this study was to investigate the antiobesity activity of this legume in mature 3T3-L1 adipocytes and in rat white adipose tissue in an ex vivo model. Mature adipocytes 3T3-L1 and rat adipose tissue were treated with bean extracts. We quantified lipolysis in mature 3T3-L1 adipocytes and in rat white adipose tissue in an ex vivo model. In an ex vivo assay with adipose tissue, methanolic and aqueous green bean extracts increased glycerol release to the medium compared to control (p < 0.05 and p < 0.001 respectively). We found that bean extracts has an antiadipogenic effect during the differentiation of 3T3L1 preadipocytes. ese results suggest that bean is a good candidate for the development of functional ingredients that can help reduce the high rates of death from cardiovascular diseases associated with obesity

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Discussion

Conclusion