Effects of perfluorooctanoic acid on Microcystis aeruginosa: Stress and self-adaptation mechanisms.

Abstract

The persistent organic pollutant perfluorooctanoic acid (PFOA) is ubiquitous in aquatic environments. However, little is known about its toxicity to microalgae or the mechanisms by which they may self-adapt to it. We found that growth of the bloom-forming cyanobacterium Microcystis aeruginosa was initially inhibited, with inhibition attenuated after 12 d of PFOA exposure. Growth inhibition gradually decreased and stabilized over time. With increasing PFOA concentration, reactive oxygen species levels and superoxide dismutase and photosystem II activity significantly increased, while respiration, NDH-1 activity, and total carbohydrate content significantly decreased. Self-adaptation mechanisms included antioxidant pathways, energy transfer and distribution of photosystems, and repair of the PSI and NDH complexes. The patterns of change in these parameters were consistent with those of the expression levels of genes in their associated metabolic pathways. Our data suggest that PSII overcompensation might be a strategy by which M. aeruginosa contends with oxidative stress induced by PFOA. Multiple downstream photosynthesis-related proteins were upregulated as a function of PFOA exposure time. These findings may help elucidate physiological, genetic stress and self-adaptive responses of microalgae to PFOA exposure.