Abstract
Simple SummaryThe intestinal microbial community in horses is very complex and interacts closely with diets. Apart from traditional forage diets, such as hay and pasture grass, silage is used to feed horses in China and other areas of the world for economic reasons or convenience of storage. Additionally, silage is also used for its convenience of harvesting and its nutrient components, including lactic acid and volatile fatty acids. In this study, we detected the characteristic composition of a fecal microbial community in horses that were fed silage with the use of a relatively new sequencing technique; we compared this result with that from horses that were fed hay and pasture grass. This study revealed some characteristic findings on the fecal microbial composition in horses that were given each of type of diet and showed significant differences between the groups. Our results provided novel data about the fecal microbial composition in horses on the silage diet. We hope that these could help balance the intestinal microbiota in horses that are mainly fed silage in combination with other types of forages in order to maintain intestinal health.Diet is an important factor affecting intestinal microbiota in horses. Fecal microbiota is commonly used as a substitute for studying hindgut microbiota when investigating the relationship between intestinal microbial changes and host health. So far, no study has compared the difference between the fecal microbiota found in horses that are fed pasture grass, silage, and hay. The present study aims to characterize the fecal microbiota in horses that were exclusively on one of the three forage diets, and to analyze the potential impact of these forages, especially silage, on horse intestinal health. There were 36 horses randomly assigned to each of the three groups; each group was fed only one type of forage for 8 weeks. High throughput sequencing was applied to analyze the bacterial taxa in fecal samples collected from the horses at the end of the feeding trial. The Lachnospiraceae family was statistically more abundant in horses fed with hay, while it was the least abundant in horses fed with silage. The Streptococcaceae spp., considered a core microbial component in equine intestinal microbiota, were present in significantly lower quantities in feces from horses that were fed pasture grass as compared to those from horses fed hay or silage. The novel data may help promote the balancing of horse intestinal microbiota and the maintenance of intestinal health in horses.
Highlights
Horses (Equus caballus) are hindgut fermenters characterized by a complex microbiota, mostly comprising anaerobic microorganisms that facilitate the digestion of a high-fiber diet [1,2,3]
We investigated the effects of three different forage feeds—grass at pasture, silage, and hay—on the horse fecal microbiota
The body condition score of all horses did not change and their body weight remained relatively stable throughout the feeding trial
Summary
Horses (Equus caballus) are hindgut fermenters characterized by a complex microbiota, mostly comprising anaerobic microorganisms that facilitate the digestion of a high-fiber diet [1,2,3]. The intestinal microbiota impacts the host’s immune system, influences the animal’s metabolism, and helps in the detoxification of harmful substances [4,5]. The disruption of an animal’s intestinal microbiota can have major consequences on its overall health. The intestinal microbiota in mammals is influenced by many external factors, including lifestyle, environment [6], as well as dietary patterns [7]. The fecal microbiota has been commonly used to study the effects of diet on the equine intestinal microbiota [7,9,10]. Diet has a significant impact on the intestinal microbiota [11]
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