Abstract
The effects of size and porosity of particles on magnetic immunoassay in a thin channel were studied. Experimental parameters were investigated and compared using a model immunoassay complex of carcinoembryonic antigen (CEA)/anti-CEA. The rate constant for the affinity reaction between functional particles increased as the size of magnetic nanoparticles (800–80nm) decreased. The affinity reaction between functional particles had no significant effect on the sizes of microparticles (1.0–4.4μm) at commonly used thin channel flow-rates of 0.001–0.025ml/min. Competitive and sandwich reactions of CEA/anti-CEA were studied for CEA detection. Microparticles of different porosities produced similar linear ranges of detection and limits of detection. The limits of detection for CEA were 0.29pg/ml and 0.21pg/ml for competitive and sandwich reactions, respectively. The linear ranges of detection were from 0.49pg/ml to 4.9ng/ml for both competitive and sandwich reactions. The detection limits were lower, and the linear ranges were wider than those of literature. There was a 9% difference in CEA detection measurements between competitive and sandwich magnetic immunoassay. The measurements of two magnetic immunoassays differed by less than 13% from the ELISA reference measurements. The running time was less than 30min. Magnetic immunoassay in a thin channel has great potential for biochemical analysis and immunoassay-related applications.
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