Effects of partially and more highly purified platelet-derived growth factor preparations on luteinizing hormone receptor induction in granulosa cell cultures.

Abstract

The effects of partially and more highly purified platelet-derived growth factor (PDGF) preparations on luteinizing hormone (LH) receptor induction by follicle-stimulating hormone (FSH) and cholera toxin (CTX) were studied in cultured granulosa cells from immature, diethylstilbestrol-primed rats. Partially purified PDGF, prepared by carboxymethyl Sephadex C-50 chromatography (CMS-PDGF), and more highly purified PDGF, further purified by Cibacron Blue Sepharose chromatography (BS-PDGF), enhance FSH-dependent LH receptor induction in serum-free and in serum-containing medium. BS-PDGF is more potent than CMS-PDGF, and is relatively more efficacious in serum-free and less efficacious in serum-containing medium than CMS-PDGF. CMS-PDGF and BS-PDGF enhance LH receptor induction by CTX in serum-containing medium, but levels achieved are significantly less than those attained with FSH and CMS- or BS-PDGF. BS-PDGF does not enhance induction by CTX in serum-free medium. The results suggest that the action of PDGF to enhance LH receptor induction is complex and may represent actions of several components of PDGF preparations. These findings may also provide indirect evidence for a component of FSH action which is independent of cAMP.