Effects of PARP-inhibitor (HO3089) and SOD-mimetic (H2545) in bilateral common carotid artery occlusion induced retinal degeneration

Abstract

Event Abstract Back to Event Effects of PARP-inhibitor (HO3089) and SOD-mimetic (H2545) in bilateral common carotid artery occlusion induced retinal degeneration Krisztina Szabadfi1*, Tamas Atlasz1, Dóra Reglődi2, Peter Kiss2, Laszlo Mester3, Robert Gabriel1, Balazs Sumegi3, Marius Opsahl2, Narve Braaten2, Aliz Szabo3, Ferenc Gallyas3 and Krisztina J. Kovacs3 1 Department of Experimental Zoology and Neurobiology, University of Pecs, Hungary 2 Department of Anatomy, Medical School, University of Pecs, Hungary 3 Department of Biochemistry and Medical Chemistry, University of Pecs, Hungary Poly (ADP-ribose) polymerase (PARP) activation plays a role in the pathogenesis of various cardiovascular, inflammatory and neurological diseases. Pharmacological inhibitors of PARP attenuate ischemic and inflammatory injury and reduce brain damage in various stroke models. Excessive generation of free radicals and decreased levels of the antioxidant enzymes such as superoxide dismutase (SOD) and catalase have been observed after brain ischemia/reperfusion injury. SOD has been shown to play a protective role in many pathological states: inflammatory diseases, ischemia/reperfusion, neurodegenerative disorders and cancer. In the present study, we investigated the neuroprotective potential of HO3089 (PARP-inhibitor) and H2545 (SOD-mimetic) in BCCAO-induced retinal degeneration. Two-month-old rats were subjected to BCCAO and their retinas were processed for histology after 3 weeks survival. We measured the number of the cells /100 µm in the ganglionic cell layer, and the thickness of outer nuclear-, outer plexiform-, inner nuclear-, and inner plexiform layers. Outer limiting membrane-inner limiting membrane distances were also examined. BCCAO resulted in severely reduced thickness of retinal layers three weeks after ligation. Neuroprotective effects of HO3089 were observed in ischemic retinal degeneration. Intraocular HO3089 treatment led to a nearly intact appearance of the retinal layers. In contrast, administration of a single dose of H2545 produced no significant neuroprotection in BCCAO-model. Our results may have clinical implications in reducing ischemic retinal degeneration in ophthalmic diseases. Support:OTKA:K72592;F67830;78480;T061766;Richter Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Pathophysiology and neurology - degenerative disorders Citation: Szabadfi K, Atlasz T, Reglődi D, Kiss P, Mester L, Gabriel R, Sumegi B, Opsahl M, Braaten N, Szabo A, Gallyas F and Kovacs KJ (2009). Effects of PARP-inhibitor (HO3089) and SOD-mimetic (H2545) in bilateral common carotid artery occlusion induced retinal degeneration. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.174 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Mar 2009; Published Online: 05 Mar 2009. * Correspondence: Krisztina Szabadfi, Department of Experimental Zoology and Neurobiology, University of Pecs, Pecs, Hungary, kriszta.szabadfi@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Krisztina Szabadfi Tamas Atlasz Dóra Reglődi Peter Kiss Laszlo Mester Robert Gabriel Balazs Sumegi Marius Opsahl Narve Braaten Aliz Szabo Ferenc Gallyas Krisztina J Kovacs Google Krisztina Szabadfi Tamas Atlasz Dóra Reglődi Peter Kiss Laszlo Mester Robert Gabriel Balazs Sumegi Marius Opsahl Narve Braaten Aliz Szabo Ferenc Gallyas Krisztina J Kovacs Google Scholar Krisztina Szabadfi Tamas Atlasz Dóra Reglődi Peter Kiss Laszlo Mester Robert Gabriel Balazs Sumegi Marius Opsahl Narve Braaten Aliz Szabo Ferenc Gallyas Krisztina J Kovacs PubMed Krisztina Szabadfi Tamas Atlasz Dóra Reglődi Peter Kiss Laszlo Mester Robert Gabriel Balazs Sumegi Marius Opsahl Narve Braaten Aliz Szabo Ferenc Gallyas Krisztina J Kovacs Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.