Effects of p38 MAPK signal pathway inhibition on upper lip scar hyperplasia at different time in rabbits

Abstract

Objective To investigate the effect of p38MAPK gene silencing recombinant adenovirus on the expression of target gene in different time and to detect the effect of p38MAPK signal pathway on the upper lip scar hyperplasia at different time to determine the optimal scar treatment time. Methods The adenovirus vector was injected into the scar tissue in 0 week, 1 week and 2 week after cheiloplasty in rabbit. The specimens were harvested in 3 week postoperatively. Four methods including Sirius red staining, immunohistochemical staining (IHC), Western blotting (WB), real-time PC (RT-PCR) were used to quantitatively and quantitatively detect the relative expression levels of p38MAPK and scar-related factors (colⅠ, colⅢ, MMP1, TIMP1). Results Sirius red staining and immunohistochemical staining showed that in 1st week the expression of colⅢ and MMP1 in scar tissue was significantly higher than that in 0 week and 2 week after operation and the expression of colⅠ and TIMP1 was significantly less than that in 0 week and 2 week after operation. The results of WB and RT-PCR were consistent with that of IHC. Conclusions After injection into the upper lip scar tissue with adenovirus in 1 week, the degree of scar hyperplasia is the least. Key words: Rabbits; Cleft lip; Cicatrix, hypertrophic; p38MAPK signaling pathway; Gene silencing; Adenovirus vector