Effects of oxytetracycline exposure in Oncorhynchus mykiss: oxidative defence system, peroxidative damage and neurotoxicity

Abstract

Event Abstract Back to Event Effects of oxytetracycline exposure in Oncorhynchus mykiss: oxidative defence system, peroxidative damage and neurotoxicity Sara Rodrigues1*, Sara C. Antunes1, Alberto T. Correia2 and Bruno S. Nunes3 1 FCUP and CIIMAR, Portugal 2 UFP and CIIMAR, Portugal 3 CESAM and DBio, Portugal In recent years, the presence and effects of pharmaceutical drugs in aquatic ecosystems has received increasing attention from the scientific community. The increasing use of pharmaceutical drugs, such as antibiotics, is cause of concern due to their potential biochemical and physiological deleterious effects. Antibiotics are particularly important because they include a variety of substances widely used in medical and veterinary practice, livestock production and aquaculture. These compounds, such as oxytetracycline (OXY), may act not only on cultured organisms, but also in non-target species. OXY is tetracycline, being used worldwide in aquacultures, due to its high efficacy against bacterial diseases (e.g. vibriosis, enteric redmouth, and also furunculosis). The present study aimed to assess the toxic effects of OXY in the freshwater fish Oncorhynchus mykiss. Fish were exposed during 96 h to OXY in concentrations of 0.005, 0.050, 0.500, 5.00 and 50.0 mg/L. In order to evaluate OXY effects in the rainbow trout, biochemical markers were analyzed, include those focused on oxidative stress parameters [catalase (CAT), total glutathione peroxidase (GPx), glutathione reductase (GRed), and glutathione-S-transferases (GSTs) activities); lipid peroxidation levels (TBARS levels), in liver and gills]; and neurotransmission, (acetylcholinesterase AChE, in muscle and eyes). The here-obtained data showed the occurrence of oxidative stress, reflected by an increased activity of GPx, GRed and GSTs (in gills) and increase of TBARS levels in liver. Additionally, it was possible to observe significant alterations in AChE activities, with decreases in the eyes and increases in muscle. Short-term effects of antibiotics were observed indicating that physiological impairment in fish may occur, with the involvement of multiple organs and biochemical pathways. The global significance of the entire set of results is discussed, giving emphasis to the ecological relevance of the responses. Keywords: Oxytetracycline, rainbow trout, Oxidative Stress, Lipid Peroxidation, neurotransmission Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Poster Presentation Topic: Physiology, Behavior and Toxicology Citation: Rodrigues S, Antunes SC, Correia AT and Nunes BS (2015). Effects of oxytetracycline exposure in Oncorhynchus mykiss: oxidative defence system, peroxidative damage and neurotoxicity. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.FMARS.2015.03.00202 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 07 Dec 2015; Published Online: 07 Dec 2015. * Correspondence: Dr. Sara Rodrigues, FCUP and CIIMAR, Porto, Portugal, up201208875@fc.up.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract Supplemental Data The Authors in Frontiers Sara Rodrigues Sara C Antunes Alberto T Correia Bruno S Nunes Google Sara Rodrigues Sara C Antunes Alberto T Correia Bruno S Nunes Google Scholar Sara Rodrigues Sara C Antunes Alberto T Correia Bruno S Nunes PubMed Sara Rodrigues Sara C Antunes Alberto T Correia Bruno S Nunes Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.