Abstract
The effects of oxygen partial pressure ( pO 2), within the range 10.6–36.5 kPa, on high density cell cultures of Panax notoginseng were investigated in a bioreactor. A pO 2 of 21.3–29.3 kPa was found optimal for the production of cell mass, ginseng saponin and polysaccharide with corresponding values of 24, 1.75 and 3.0 g l −1 on Day 15, respectively. A low pO 2 was unfavorable to the cell cultures due to oxygen limitation. High pO 2 (36.5 kPa) inhibited cell growth and reduced the production of ginseng saponin and polysaccharide, which was due to the detrimental effect of oxidative burst. The effect of pO 2 on heterogeneity of ginsenosides produced in the cell cultures was also studied. A high pO 2 (at 36.5 kPa) stimulated Rb 1 biosynthesis in the initial stage of cultivation, while a low pO 2 (at 10.6 kPa) inhibited Rg 1 and Rb 1 formation during cultivation.
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