Abstract

Oviducal epithelial cells were cultured and the effects of the cells on the survival and fertilizing ability of fowl spermatozoa were investigated. When spermatozoa were incubated at 41 degrees C with cells from the infundibulum, shell gland or uterovaginal junction, their motility, assessed at room temperature (20-23 degrees C), was maintained for 5 to 6 days. Fertilizing ability was also retained after culture at 41 degrees C for at least 4 days. During incubation at this temperature, the spermatozoa were almost immotile and rested in close proximity to the oviducal cells. At 23 degrees C, the same spermatozoa were highly motile.

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