Abstract
Objective To discuss the overexpression of macrophage migration inhibitory factor (MIF) on bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) in osteoblasts.Methods Human osteoblasts were isolated from 5 patients,and then pcDNA3.0-MIF was constructed and transfected into osteoblasts.The effect of MIF overexpression on BMP-2 and VEGF in osteoblasts was detected.Results MIF overexpression was verified (P ＜0.05).MIF,and BMP-2 and VEGF mRNA and protein levels were higher in transfected cells than in control cells (P ＜ 0.05).Conclusion Overexpression of MIF up-regulated the BMP-2 and VEGF in osteoblasts. Key words: Osteoblast cells; Macrophage migration inhibitory factor; Bone morphogenetic protein 2; Vascular endothelial growth factor
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