Effects of ouabain on hypoxia-induced ANP release in beating rabbit atria

Abstract

Objective To confirm the effects of hypoxia on the atrial ANP secretion and to determine the relationship between ouabain and hypoxia induced ANP release. Methods Experiments were performed in a perfused beating rabbit atrial model and thirty New Zealand white rabbits were used. Hypoxic atrial model was produced by Nitrogen gas. While setting up the model, atria were perfused for 60min and the control period (12min as an experimental cycle) was followed by different series of experiments simultaneously atrial dynamics were monitored.