Abstract
The effects of osmotic pressure were investigated on cell growth and von willebrand factor (vWF) expression in batch culture, pulse culture and adaptive culture of recombinant baby hamster kidney (rBHK) cells. Intracellular contents of some amino acids including aspartic acid, glycine, arginine, alanine, valine and serine in adaptive culture showed a significant increase with environmental osmotic pressure and became steady after 6 h adaptation. There was little change in intracellular concentrations of amino acids in a control cultivation under 330 mOsmol kg −1. With the increase of osmotic pressure from 330 to 350 mOsmol kg −1, the specific growth rate of rBHK cells remained kept constant. However, the growth of rBHK cells was seriously inhibited under 370 mOsmol kg −1. When gradually increasing the osmotic pressure from 370 to 470 mOsmol kg −1 over more than 6 h, the specific growth rate of rBHK cells could increase by 40% in comparison with that when directly increasing within the same range. High osmotic pressure hardly effected any change in the percent of both cells during G 0/G 1 phase and apoptotic cells in the cell population, but the percentage of cells during S phase in the cell population increased. Higher osmotic pressure (470 mOsmol kg −1) could inhibit the expression of vWF, although at 370 mOsmol kg −1 the specific production rate of vWF was 47% higher than that in 330 mOsmol kg −1.
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