Effects of orthodontic force magnitude on cell apoptosis and RANKL-induced osteoclastogenesis : Studies in arat model.

Abstract

The aim of this study was to evaluate the time course of orthodontic force-induced apoptosis and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis in arat model under light- and heavy-force conditions. Male Wistar rats were divided into light-force (10 cN) and heavy-force (60 cN) groups (N = 28/group). Each group was divided into four time-course subgroups to evaluate all phases of orthodontic tooth movement. Mesialization appliances were placed on three united maxillary molars unilaterally and activated. Tooth movements were calculated, and periodontal ligament (PDL) widths were measured. Expression of Bax, Bcl‑2, caspase3, caspase9, and RANK-RANKL were assessed by immunohistochemistry. Expression levels at the PDL-alveolar bone border were compared between experimental and control groups and force groups. The rate of tooth movement did not differ between the force groups. PDL widths were higher on the tension side in the heavy-force group in the post-lag phase. Pro-apoptotic protein Bax expression was elevated in the heavy-force group, whereas anti-apoptotic protein Bcl‑2 expression was elevated in the light-force group. RANK expression on days7 and21 and RANKL expression on day21 differed between the force groups. Evidence of orthodontic force-induced apoptosis is more robust with stronger forces than with weaker forces. Exuberant RANKL-induced osteoclastogenesis that was seen when applying alow force results from increased RANK and RANKL expression in the post-lag phase.