Effects of organic chemical quality and mineral nitrogen addition on lignin and holocellulose decomposition of beech leaf litter by Xylaria sp.

Abstract

Mycobiota and chemical composition of bleached and non-bleached portions were studied on leaf litter of beech ( Fagus crenata Blume). By surface sterilization method, two xylariaceous species Xylaria sp. and Geniculosporium sp.1 were dominantly isolated in both portions. Frequency of occurrence of Xylaria sp. was significantly higher in the bleached portion than in the non-bleached portion. In the bleached portion, lignin concentration was lower than in the non-bleached portion, indicating that Xylaria sp. and Geniculosporium sp.1 took part in lignocellulose decomposition in the study site. Effects of organic chemical quality of litters and exogenous mineral nitrogen (NH 4 and NO 3) addition were then investigated on in vitro lignin decomposition by Xylaria sp. Weight loss of lignin was significantly related to lignocellulose index (LCI) for four litter types tested. In NH 4 and NO 3 addition treatments, lignin decomposition was completely and partially suppressed, respectively. Xylaria sp. produced bleaching spots on beech leaf litter in vitro in which lignin concentration was lower than in the non-bleached portion. These results suggest that heterogeneous distribution of carbon and nitrogen resources may control lignin decomposition on the litter by the fungus.