Effects of organic carbon sources on the biomass and lipid production by the novel microalga Micractinium reisseri FM1 under batch and fed-batch cultivation

Abstract

A microalgal strain Micractinium reisseri FM1 which could assimilate organic carbon sources effectively and grow rapidly under mixotrophic condition was screened and identified through morphological and 18S rRNA gene sequence. Addition of glucose, maltose and sodium acetate at 2 and 4 g L−1 contents could promote the biomass production, lipid content and productivity significantly. Compared with batch culture (8 g L−1), fed-batch culture (fed 2 g L−1 4 times) with glucose, maltose and sodium acetate respectively, could not only enhance biomass, but also boost lipid productivity. More importantly, the composition of fatty acid could be enriched and the proportion of saturated to unsaturated fatty acids could be improved on the basis of promoting alga growth and lipid production by feeding organic carbon and regulating culture mode. M. reisseri FM1 has the potential to assimilate organic carbon sources for high-cell density cultivation and production of biodiesel or functional polyunsaturated fatty acids.