Effects of Oral Glucosamine Hydrochloride Administration on Plasma Free Amino Acid Concentrations in Dogs

Kazuo Azuma,Saburo Minami,Tomohiro Imagawa,Tomohiro Osaki,Yoshiharu Okamoto,Takeshi Tsuka,Yoshimori Takamori

doi:10.3390/md9050712

Kazuo Azuma, Saburo Minami + Show 5 more

Open Access

https://doi.org/10.3390/md9050712

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Journal: Marine Drugs	Publication Date: Apr 27, 2011
Citations: 22	License type: CC BY 3.0

Affiliation: Tottori University, Koei Chemical (Japan)

Abstract

We examined the effects of oral glucosamine hydrochloride (GlcN), N-acetyl-d-glucosamine (GlcNAc) and d-glucose (Glc) administration on plasma total free amino acid (PFAA) concentrations in dogs. The PFAA concentrations increased in the control group and the GlcNAc group at one hour after feeding, and each amino acid concentration increased. On the other hand, in the GlcN group and the Glc group PFAA concentrations decreased at one hour after feeding. A significant decrease in amino acid concentration was observed for glutamate, glycine and alanine. Our results suggest the existence of differences in PFAA dynamics after oral administration of GlcN and GlcNAc in dogs.

Highlights

Glucosamine hydrochloride (GlcN) and N-acetyl-D-glucosamine (GlcNAc) are components of glycosaminoglycan widely used as dietary supplements [1]
The plasma total free amino acid (PFAA) concentrations increased in the control dogs and the GlcNAc treated dogs whereas those from the GlcN- or the Glc-treated dogs significantly decreased after one hour (Figure 1)
The levels of Glu, Gly, and Ala concentrations were significantly lower than observed fort he GlcNAc-treated dogs (Table 2)

Summary

Introduction

Glucosamine hydrochloride (GlcN) and N-acetyl-D-glucosamine (GlcNAc) are components of glycosaminoglycan widely used as dietary supplements [1]. GlcN has the potential to exert chondroprotective action on an experimentally induced osteoarthritis by inhibiting type II collagen degradation and enhancing type II collagen synthesis in the articular cartilage [13]. These results suggested that synthesis of type II collagen and proteoglycan core protein as well as glycosaminoglycan occurs upon GlcN and GlcNAc supplementation. We investigated (PFAA) dynamics after oral administration of GlcN, GlcNAc, or glucose (Glc), which are the sources of glycosaminoglycan and proteoglycan in the body

Results and Discussion

Materials

Administration and Blood Sampling

Measurement of PFAA Concentrations

Measurement of Plasma GlcN and GlcNAc Concentrations

Statistical Analysis

Conclusions