Effects of Ontogeny on δ13C of Plant- and Soil-Respired CO2 and on Respiratory Carbon Fractionation in C3 Herbaceous Species.

Yann Salmon,Nina Buchmann,Romain L Barnard

doi:10.1371/journal.pone.0151583

Abstract

Knowledge gaps regarding potential ontogeny and plant species identity effects on carbon isotope fractionation might lead to misinterpretations of carbon isotope composition (δ13C) of respired CO2, a widely-used integrator of environmental conditions. In monospecific mesocosms grown under controlled conditions, the δ13C of C pools and fluxes and leaf ecophysiological parameters of seven herbaceous species belonging to three functional groups (crops, forage grasses and legumes) were investigated at three ontogenetic stages of their vegetative cycle (young foliage, maximum growth rate, early senescence). Ontogeny-related changes in δ13C of leaf- and soil-respired CO2 and 13C/12C fractionation in respiration (ΔR) were species-dependent and up to 7‰, a magnitude similar to that commonly measured in response to environmental factors. At plant and soil levels, changes in δ13C of respired CO2 and ΔR with ontogeny were related to changes in plant physiological status, likely through ontogeny-driven changes in the C sink to source strength ratio in the aboveground plant compartment. Our data further showed that lower ΔR values (i.e. respired CO2 relatively less depleted in 13C) were observed with decreasing net assimilation. Our findings highlight the importance of accounting for ontogenetic stage and plant community composition in ecological studies using stable carbon isotopes.

Highlights

The carbon isotopic composition (δ13C) of plant- and soil-respired CO2 is often used to infer plant physiological responses and compare the responses of plants and ecosystems to changes in environmental conditions (e.g., [2])
The present study focused on the effect of ontogeny and species identity on the isotopic signature of respired CO2 and the associated ΔR
This experiment included seven C3 herbaceous species covering three different functional groups, based on a priori plant biological characteristics related to C and nitrogen allocation strategies: Arrhenatherum elatius L., Dactylis glomerata L., Lolium perenne L., Hordeum vulgare L., Triticum aestivum L., Medicago sativa L., Trifolium pratense L

Summary

Introduction

The carbon isotopic composition (δ13C) of plant- and soil-respired CO2 is often used to infer plant physiological responses (e.g., review by [1]) and compare the responses of plants and ecosystems to changes in environmental conditions (e.g., [2]). The effects of ontogeny and plant species identity on respiratory signatures in the plant-soil continuum remain poorly understood. These biological factors are often difficult to PLOS ONE | DOI:10.1371/journal.pone.0151583. Differentiating biological from environmental effects on the isotopic signature of plant-soil continuum components requires controlling either one or the other. To date, this has been tackled in only a few studies under controlled environmental conditions. It is crucial to understand the interaction between ontogeny and species identity when studying changes in isotopic signature of plant-soil continuum

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